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Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity

Raza, Waseem, Yang, Wang, Jun, Yuan, Shakoor, Faheem, Huang, Qiwei, Shen, Qirong
Carbohydrate polymers 2012 v.90 no.2 pp. 921-929
Pseudomonas fluorescens, antioxidant activity, arabinose, free radical scavengers, glucose, hydrogen peroxide, maltose, manganese, models, molecular weight, pH, peptones, polysaccharides, response surface methodology, temperature, uronic acids, zinc
The extracellular polysaccharide produced by a newly isolated strain Pseudomonas fluorescens WR-1 was purified and characterized and its production was optimized using response surface methodology. The results showed that the strain WR-1 produced one kind of EPS that was composed of arabinose, glucose and uronic acid. The molecular weight of the EPS was determined to be 6.78×10⁶Da. The preferable culture conditions for EPS production were pH 7.0, temperature 28°C for 72h with peptone and maltose as best N and C sources, respectively. The model predicted that the maximum EPS production (39.6gL⁻¹) was appeared with maltose 48.65gL⁻¹, Mn²⁺ 1118μM and Zn²⁺ 901μM. The EPS also showed good H₂O₂ scavenging activity while moderate free radical scavenging activity and reductive ability were determined. The EPS from WR-1 may be a new source of natural antioxidants with potential value for health, food and industry.