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Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity
- Raza, Waseem, Yang, Wang, Jun, Yuan, Shakoor, Faheem, Huang, Qiwei, Shen, Qirong
- Carbohydrate polymers 2012 v.90 no.2 pp. 921-929
- Pseudomonas fluorescens, antioxidant activity, arabinose, free radical scavengers, glucose, hydrogen peroxide, maltose, manganese, models, molecular weight, pH, peptones, polysaccharides, response surface methodology, temperature, uronic acids, zinc
- The extracellular polysaccharide produced by a newly isolated strain Pseudomonas fluorescens WR-1 was purified and characterized and its production was optimized using response surface methodology. The results showed that the strain WR-1 produced one kind of EPS that was composed of arabinose, glucose and uronic acid. The molecular weight of the EPS was determined to be 6.78×10⁶Da. The preferable culture conditions for EPS production were pH 7.0, temperature 28°C for 72h with peptone and maltose as best N and C sources, respectively. The model predicted that the maximum EPS production (39.6gL⁻¹) was appeared with maltose 48.65gL⁻¹, Mn²⁺ 1118μM and Zn²⁺ 901μM. The EPS also showed good H₂O₂ scavenging activity while moderate free radical scavenging activity and reductive ability were determined. The EPS from WR-1 may be a new source of natural antioxidants with potential value for health, food and industry.