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Structural studies of the pectic polysaccharide from Siberian fir (Abies sibirica Ledeb.)

Author:
Makarova, Elena N., Patova, Olga A., Shakhmatov, Evgeny G., Kuznetsov, Sergey P., Ovodov, Yury S.
Source:
Carbohydrate polymers 2013 v.92 no.2 pp. 1817-1826
ISSN:
0144-8617
Subject:
Abies sibirica, acid hydrolysis, esters, hydrochloric acid, ion exchange chromatography, nuclear magnetic resonance spectroscopy, pH, polysaccharides, sugars, wood
Abstract:
The pectic polysaccharide named abienan AS-A was isolated from the wood greenery of Abies sibirica using dilute hydrochloric acid (pH 4.0) at 70°C. The structure of abienan AS-A was elucidated using sugar composition analysis, ion-exchange chromatography and partial acid hydrolysis followed by NMR spectroscopy. The linear region of abienan AS-A was shown to contain linear 1,4-α-d-galactopyranosyluronan partially substituted with methyl esters or 3-O-acetyl groups and rhamnogalacturonan blocks consisting of 1,4-α-d-galacturonan partially substituted with methyl ester groups and connected by 2-O-substituted α-rhamnopyranose residues. The branched region of abienan AS-A was found to be made of RG-I. The side chains of RG-I were shown to contain 1,4-β-galactan and branched arabinan. Some 4-O-substituted β-galactopyranose residues were shown to be attached to the 4-position of the 2-O-substituted α-rhamnopyranose residues of the RG-I backbone. The arabinan groups were made up of a 1,5-linked α-l-arabinofuranan backbone that was 3-O-, 2-O-, and 2,3-di-O-substituted with the terminal and 1,3-linked α-l-arabinofuranose residues.
Agid:
880161