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A Cryptic Wheat–Aegilops triuncialis Translocation with Leaf Rust Resistance Gene Lr58

Kuraparthy, Vasu, Sood, Shilpa, Chhuneja, Parveen, Dhaliwal, Harcharan S., Kaur, Satinder, Bowden, Robert L., Gill, Bikram S.
Crop science 2007 v.47 no.5 pp. 1995
Triticum aestivum, wheat, Aegilops triuncialis, wild relatives, plant genetic resources, Puccinia recondita, plant pathogenic fungi, rust diseases, disease resistance, genetic resistance, nucleic acid hybridization, gene segregation, chromosome mapping, inheritance (genetics), alleles, translocation lines, restriction fragment length polymorphism, genetic markers, India, Kansas
Genes transferred to crop plants from wild species are often associated with deleterious traits. Using molecular markers, we detected a cryptic introgression with a leaf rust resistance gene transferred from L. into common wheat (L.). One agronomically desirable rust-resistant introgression line was selected and advanced to BCF from a cross of hexaploid wheat and In situ hybridization using genomic DNA as a probe failed to detect the alien introgression. The translocation line was resistant to the most prevalent races of leaf rust in India and Kansas. Genetic mapping in a segregating F population showed that the rust resistance was monogenically inherited. Homeologous group 2 restriction fragment length polymorphism markers , , and showed diagnostically polymorphic alleles between the resistant and susceptible bulks. The alien transfer originated from homeologous chromosome recombination. The -specific alleles of , , , and one simple sequence repeat marker cosegregated with the rust resistance, suggesting that the wheat– translocation occurred in the distal region of chromosome arm 2BL. This translocation was designated T2BS·2BL-2L(0.95). The unique source and map location of the introgression on chromosome 2B indicated that the leaf rust resistance gene is new and was designated