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A Practical Guide to the Isolation, Analysis and Identification of Conjugated Linoleic Acid
- Christie, William W., Dobson, Gary, Adlof, Richard O.
- Lipids 2007 v.42 no.12 pp. 1073
- conjugated linoleic acid, isomers, free fatty acids, triacylglycerols, chemical analysis, product authenticity, gas chromatography, high performance liquid chromatography, reversed-phase high performance liquid chromatography, mass spectrometry, nuclear magnetic resonance spectroscopy
- Natural and synthetic conjugated linoleic acids (CLA) are reputed to have therapeutic properties that are specific to particular geometrical and positional isomers. Analysis of these has presented unique problems that have brought forward distinctive solutions, especially the use of gas chromatography-mass spectrometry and silver-ion high-performance liquid chromatography. In the analysis of CLA present at low levels in tissue samples, it is sometimes necessary to use concentration methods. In this review, the most useful and practical methods for the isolation and analysis of CLA isomers in tissues and in commercial CLA preparations are described.