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Behavioral effects of titanium dioxide nanoparticles on larval zebrafish (Danio rerio)

Chen, Te-Hao, Lin, Chun-Yao, Tseng, Mei-Chen
Marine pollution bulletin 2011 v.63 no.5-12 pp. 303-308
Danio rerio, abnormal development, acetylcysteine, endpoints, fish, glutathione, larvae, nanoparticles, oxidative stress, swimming, titanium dioxide, toxicity, water pollution
In this study, zebrafish embryos were exposed to titanium dioxide nanoparticles (TiO₂ NPs at 0.1, 0.5, 1, 5, 10mg/L or control) from fertilization to free swimming stage. Hatchability, survival, and malformation rate were not affected by TiO₂ NPs at these exposure levels. However, larval swimming parameters, including average and maximum velocity and activity level were significantly affected by TiO₂ NPs. Co-exposure to either the glutathione precursor, N-acetylcysteine (NAC), or the glutathione synthesis inhibitor, buthionine sulfoximine (BSO), did not significantly alter the behavioral effects resulting from TiO₂ NPs, suggesting that other factor(s) besides oxidative stress may contribute to the behavioral toxicity of TiO₂ NPs. Our study also demonstrated that the behavioral endpoints were more sensitive than the others (e.g., hatchability and survival) to detect toxicity of TiO₂ NPs on developing fish.