PubAg

Main content area

Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver

Author:
Miyamura, Norio, Nakamura, Takashi, Goto-Inoue, Naoko, Zaima, Nobuhiro, Hayasaka, Takahiro, Yamasaki, Tokiwa, Terai, Shuji, Sakaida, Isao, Setou, Mitsutoshi, Nishina, Hiroshi
Source:
Biochemical and biophysical research communications 2011 v.408 no.1 pp. 120-125
ISSN:
0006-291X
Subject:
adipose tissue, genes, image analysis, lipid metabolism, liver, mass spectrometry, mice, phosphatidylcholines, reverse transcriptase polymerase chain reaction, thin layer chromatography, triacylglycerols
Abstract:
After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.
Agid:
893863