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Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats

Obata, Norihiko, Mizobuchi, Satoshi, Itano, Yoshitaro, Matsuoka, Yoshikazu, Kaku, Ryuji, Tomotsuka, Naoto, Morita, Kiyoshi, Kanzaki, Hirotaka, Ouchida, Mamoru, Yokoyama, Masataka
Biochemical and biophysical research communications 2011 v.408 no.1 pp. 139-144
analgesics, exons, ganglia, gene expression regulation, messenger RNA, models, nerve tissue, neurons, pain, promoter regions, rats, reverse transcriptase polymerase chain reaction, spinal cord
The mechanism underlying neuropathic pain is still largely unclear. Recently, much attention has been focused on the role of brain-derived neurotrophic factor (BDNF) as a neuromodulator in the spinal cord. We previously reported that the expression of Bdnf exon I mRNA was remarkably up-regulated in the dorsal root ganglion (DRG) neurons with the rat L5 spinal nerve ligation (SNL) model. In the present study, we investigated whether neuropathic pain response would be reduced by the inhibition of the Bdnf exon I in the rat SNL model. We identified the promoter region of exon I and synthesized the decoy ODNs targeting the region. Reverse transcription-polymerase chain reaction analysis confirmed that the decoy ODN treatment reduced SNL-induced Bdnf exon I mRNA up-regulation in ipsilateral L4 and L5 DRGs. Furthermore, post-treatment with the decoy ODNs significantly attenuated SNL-induced tactile allodynia. This study suggested that decoy ODNs targeting the Bdnf exon I might provide a novel analgesic strategy for the treatment of neuropathic pain.