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New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase

Author:
Senoura, Takeshi, Ito, Shigeaki, Taguchi, Hidenori, Higa, Mariko, Hamada, Shigeki, Matsui, Hirokazu, Ozawa, Tadahiro, Jin, Shigeki, Watanabe, Jun, Wasaki, Jun, Ito, Susumu
Source:
Biochemical and biophysical research communications 2011 v.408 no.4 pp. 701-706
ISSN:
0006-291X
Subject:
Bacteroides, Cellvibrio, Flavobacterium, glycolysis, microorganisms, operon, phosphorylase, sugars
Abstract:
The consecutive genes BF0771–BF0774 in the genome of Bacteroidesfragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-β-d-mannopyranosyl-d-glucose+Pi→mannose-1-phosphate+glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-β-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and Cellvibrio.
Agid:
893949