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Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells

Author:
Kageyama-Yahara, Natsuko, Wang, Xiaoyu, Katagiri, Tatsuo, Wang, Ping, Yamamoto, Takeshi, Tominaga, Makoto, Kadowaki, Makoto
Source:
Biochemical and biophysical research communications 2011 v.416 no.3-4 pp. 283-288
ISSN:
0006-291X
Subject:
bone marrow, calcium, cortex, food allergies, immunoglobulin E, mast cells, phospholipase C, phosphorylation, tyrosine
Abstract:
Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca²⁺ level that is induced by the extracellular Ca²⁺ influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) γ1, which is a crucial activation switch for the intracellular Ca²⁺ mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca²⁺ mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLCγ-Ca²⁺ influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies.
Agid:
894951