PubAg

Main content area

Bioluminescent system for dynamic imaging of cell and animal behavior

Author:
Hara-Miyauchi, Chikako, Tsuji, Osahiko, Hanyu, Aki, Okada, Seiji, Yasuda, Akimasa, Fukano, Takashi, Akazawa, Chihiro, Nakamura, Masaya, Imamura, Takeshi, Matsuzaki, Yumi, Okano, Hirotaka James, Miyawaki, Atsushi, Okano, Hideyuki
Source:
Biochemical and biophysical research communications 2012 v.419 no.2 pp. 188-193
ISSN:
0006-291X
Subject:
Aequorea, animal behavior, bioluminescence, cell movement, drugs, fluorescent proteins, genetically modified organisms, image analysis, luciferase, mice
Abstract:
The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, ffLuc-cp156, which consists of a yellow variant of Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.
Agid:
895376