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GRK5 ablation contributes to insulin resistance

Wang, Li, Shen, Minjie, Wang, Feifei, Ma, Lan
Biochemical and biophysical research communications 2012 v.429 no.1-2 pp. 99-104
G-protein coupled receptors, adipose tissue, fatty liver, gene expression, genes, glucose, glucose tolerance, high fat diet, homeostasis, insulin resistance, messenger RNA, mice, noninsulin-dependent diabetes mellitus, obesity, pathogenesis, protein-serine-threonine kinases, signal transduction, threonine, transcription factor NF-kappa B
The G-protein-coupled receptor kinase 5 (GRK5) is an important member of the threonine/serine kinase family that phosphorylates and regulates the G-protein-coupled receptor (GPCR) signaling pathway. GRK5 is highly expressed in adipose tissue and may act as an adipogenetic factor under high-fat load [1]. Insulin resistance is associated with the pathogenesis of metabolic disorders such as type 2 diabetes and obesity; however, the potential role of GRK5 in insulin resistance is unknown. We characterized the biochemical and molecular alterations related to metabolic complications observed in GRK5⁻/⁻ mice. These mice, which are partially resistant to obesity induced by a high-fat diet, had impaired glucose tolerance and insulin sensitivity, as well as disruption of AKT signaling transduction compared with their wild-type littermates. Further study showed that the decreased insulin sensitivity was not attributable to alterations in inflammatory status such as the NF-κB signaling pathway or inflammatory gene expression. Instead, hepatic steatosis and changes of mRNA in genes involved in hepatic glucose and lipid homeostasis were found. Overall, our data identified GRK5 as a positive regulator of insulin sensitivity. Our results showed that this protein is a potential therapeutic target in the treatment of insulin resistance and related disorders.