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Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method
- Hamanaka, Sanae, Ooehara, Jun, Morita, Yohei, Ema, Hideo, Takahashi, Satoru, Miyawaki, Atsushi, Otsu, Makoto, Yamaguchi, Tomoyuki, Onodera, Masafumi, Nakauchi, Hiromitsu
- Biochemical and biophysical research communications 2013 v.435 pp. 586-591
- bone marrow, embryonic stem cells, erythrocytes, erythropoiesis, fluorescent proteins, gene expression, genetically modified organisms, mice, proviruses, somatic cells
- Fluorescent-protein transgenic mice are useful for obtaining marked somatic cells to study kinetics of development or differentiation. Fluorescence-marked hematopoietic stem cells in particular are commonly used for studying hematopoiesis. However, as far as we know, no transgenic mouse line is described in which a fluorescent protein is stably and constitutively expressed in all hematopoietic cells, including erythrocytes and platelets. Using the random segregation of provirus (RSP) method, we generated from retrovirally transduced mouse embryonic stem cells a transgenic mouse line expressing a red/orange fluorescent protein, Kusabira Orange (KuO). KuO transgenic mouse line cells carry only one proviral integration site and stably express KuO in all hematopoietic-lineage elements, including erythrocytes and platelets. Moreover, bone-marrow transplantation in KuO transgenic mice demonstrated normal hematopoieisis. KuO transgenic mice likely will prove useful for study of hematopoiesis that includes erythropoiesis and megakaryopoiesis.