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The hUPF1-NMD factor controls the cellular transcript levels of different genes of complex I of the respiratory chain
- Panelli, Damiano, Lorusso, Francesca Paola, Trentadue, Raffaella, Stella, Alessandro, Sardanelli, Anna Maria, Papa, Sergio
- Biochimie 2012 v.94 no.12 pp. 2600-2607
- alternative splicing, electron transport chain, genes, messenger RNA, transcriptome
- In this study the impact of hUPF1 and hUPF2 knockdown on alternative splicing (AS) isoforms of different genes encoding subunits of respiratory chain complex I and complex IV is described. As expected, loss of both hUPF1 and hUPF2 led to impairment of nonsense-mediated mRNA decay (NMD) and accumulation of PTC-containing NMD substrates generated by both complex I and complex IV genes. The levels of some complex I splice variants, which did not contain PTC as well as the level of some complex I canonical transcripts were, however, affected only by hUPF1 knockdown. This finding confirms that NMD plays a role in the maintenance of the transcriptome integrity and reveals a specific impact of hUPF1 on the regulation of complex I genes.