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Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials

Author:
Behlau, Irmgard, Mukherjee, Koushik, Todani, Amit, Tisdale, Ann S., Cade, Fabiano, Wang, Liqiang, Leonard, Elizabeth M., Zakka, Fouad R., Gilmore, Michael S., Jakobiec, Frederick A., Dohlman, Claes H., Klibanov, Alexander M.
Source:
Biomaterials 2011 v.32 no.34 pp. 8783-8796
ISSN:
0142-9612
Subject:
Staphylococcus aureus, antibacterial properties, biocompatibility, biofilm, cornea, cytotoxicity, electron microscopy, epithelial cells, rabbits, titanium
Abstract:
The biocompatibility and antibacterial properties of N,N-hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro. Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.
Agid:
903713