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The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli
- Cui, Yan, Zhao, Yuyun, Tian, Yue, Zhang, Wei, Lü, Xiaoying, Jiang, Xingyu
- Biomaterials 2012 v.33 no.7 pp. 2327-2333
- Escherichia coli, Gram-negative bacteria, adenosine triphosphate, adenosinetriphosphatase, antibacterial properties, antibiotics, chemotaxis, death, energy metabolism, gold, mechanism of action, membrane potential, multiple drug resistance, nanogold, proteomics, reactive oxygen species, ribosomes, transcriptomics, transfer RNA
- This work examines the molecular mechanism of action of a class of bactericidal gold nanoparticles (NPs) which show potent antibacterial activities against multidrug-resistant Gram-negative bacteria by transcriptomic and proteomic approaches. Gold NPs exert their antibacterial activities mainly by two ways: one is to collapse membrane potential, inhibiting ATPase activities to decrease the ATP level; the other is to inhibit the subunit of ribosome from binding tRNA. Gold NPs enhance chemotaxis in the early-phase reaction. The action of gold NPs did not include reactive oxygen species (ROS)-related mechanism, the cause for cellular death induced by most bactericidal antibiotics and nanomaterials. Our investigation would allow the development of antibacterial agents that target the energy-metabolism and transcription of bacteria without triggering the ROS reaction, which may be at the same time harmful for the host when killing bacteria.