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Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats

Author:
WEI, Li Li, CHEN, Hong, JIANG, Yong, TU, Peng Fei, ZHONG, Ming, DU, Juan, Fei, LIU, WANG, Lei, LIU, Chun Yang
Source:
Biomedical and environmental sciences 2012 v.25 no.2 pp. 238-244
ISSN:
0895-3988
Subject:
5-hydroxyindoleacetic acid, dihydroxyphenylalanine, dopamine, electrochemistry, homovanillic acid, ischemia, males, microdialysis, neurons, norepinephrine, rats, reversed-phase high performance liquid chromatography, serotonin
Abstract:
OBJECTIVE: To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine (NE), dopamine (DA), homovanillic acid (HVA), 3, 4-dihydroxyphenylethanoid acid (DOPAC), 5-hydroxyindoleacetic acid (HIAA), and 5-hydroxytryptamine(5-HT) in middle cerebral artery occlusion (MCAO rats. METHODS: The middle cerebral artery was occluded in male Sprague-Dawley rats. Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer's solution at a rate of 1.5 µL/min. Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE, DA, HVA, DOPAC, HIAA, and 5-HT levels by reverse phase HPLC with electrochemistry. RESULTS: Three days after MCAO, the extracellular striatal levels of NE, DA, DOPAC, HIAA, HVA, and 5-HT of the MCAO rats increased significantly (at least P<0.05 vs. control). However, simultaneous treatment with echinacoside (30.0 or 15.0 mg/kg) attenuated these increases (at least P<0.05 vs. non-treated model rats). CONCLUSION: These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.
Agid:
905804