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Characterization of 57 microsatellite loci for Rapana venosa using genomic next generation sequencing
- Sun, Xiaoqi, Yu, Hong, Yu, Ruihai, Li, Qi
- Conservation genetics resources 2014 v.6 no.4 pp. 941-945
- alleles, high-throughput nucleotide sequencing, loci, microsatellite repeats
- A total of 57 polymorphic microsatellite loci were developed for Rapana venosa through 454 sequencing. The 57 loci were characterized in 30 wild individuals. The number of alleles per locus varied from 2 to 14. The observed and expected heterozygosities ranged from 0.100 to 1.000 and 0.127 to 0.919, respectively. Thirty-two loci were in Hardy–Weinberg equilibrium. The microsatellite loci described in this study will facilitate investigation of conservation genetics of this species.