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Cross amplification of 15 EST-SSR markers in the genus Fraxinus

Author:
Noakes, Andi G., Best, Teodora, Staton, Margaret E., Koch, Jennifer, Romero-Severson, Jeanne
Source:
Conservation genetics resources 2014 v.6 no.4 pp. 969-970
ISSN:
1877-7252
Subject:
Agrilus planipennis, Fraxinus pennsylvanica, forest trees, indigenous species, mortality, population dynamics, species identification, Asia, United States
Abstract:
Ash (Fraxinus, Oleaceae) species occur on most continents, within a wide range of forest tree communities. Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), introduced into the U.S. from Asia in the late twentieth century, has caused widespread mortality, primarily in green ash, Fraxinus pennsylvanica Marsh. (Section: Melioides) and now impacts other North American ash species. The development and successful reintroduction of resistant trees requires genetic tools to evaluate population dynamics and aid in species identification. Here, we report 15 novel EST-SSR markers developed in green ash, most of which amplify and are polymorphic across 18 species of Fraxinus, including six species native to North America. The high average polymorphism information content (0.741) and allelic richness (15.3) revealed in six disparate populations of green ash indicate that these markers also have utility for investigating population dynamics in this species.
Agid:
918227