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A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection
- Zhao, Huimin, Tian, Junping, Quan, Xie
- Colloids and Surfaces B: Biointerfaces 2013 v.103 pp. 38-44
- chitosan, colloids, detection limit, electrochemistry, electrodes, graphene, high performance liquid chromatography, horseradish, immunosensors, microcystin-LR, monitoring, nanospheres, water quality
- A graphene-based immunosensor was fabricated for the detection of microcystin-LR using a horseradish peroxidase–carbon nanosphere–antibody system for signal amplification. Graphene and chitosan were used as immobilization materials to improve the electrochemical performance of the electrode. Signal amplification was achieved using multienzyme functionalized carbon nanosphere. This approach provided a linear range from 0.05 to 15μgL−1 microcystin-LR with a detection limit of 0.016μgL−1. Analytical detection of microcystin-LR in environmental water samples were demonstrated by comparing the results obtained using immunosensor and high-performance liquid chromatography. This electrochemical immunosensor showed good performance with high sensitivity, acceptable stability, repeatability and the potential for use in routine water quality monitoring for various toxins.