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In vitro evaluation of paclitaxel loaded amorphous chitin nanoparticles for colon cancer drug delivery

Smitha, K.T., Anitha, A., Furuike, T., Tamura, H., Nair, Shantikumar V., Jayakumar, R.
Colloids and Surfaces B: Biointerfaces 2013 v.104 pp. 245-253
antineoplastic agents, biodegradability, chitin, colloids, colorectal neoplasms, crosslinking, drug delivery systems, drug therapy, flow cytometry, fluorescence microscopy, in vitro studies, nanoparticles, neoplasm cells, paclitaxel, toxicity
Chitin and its derivatives have been widely used in drug delivery applications due to its biocompatible, biodegradable and non-toxic nature. In this study, we have developed amorphous chitin nanoparticles (150±50nm) and evaluated its potential as a drug delivery system. Paclitaxel (PTX), a major chemotherapeutic agent was loaded into amorphous chitin nanoparticles (AC NPs) through ionic cross-linking reaction using TPP. The prepared PTX loaded AC NPs had an average diameter of 200±50nm. Physico-chemical characterization of the prepared nanoparticles was carried out. These nanoparticles were proven to be hemocompatible and in vitro drug release studies showed a sustained release of PTX. Cellular internalization of the NPs was confirmed by fluorescent microscopy as well as by flow cytometry. Anticancer activity studies proved the toxicity of PTX-AC NPs toward colon cancer cells. These preliminary results indicate the potential of PTX-AC NPs in colon cancer drug delivery.