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In vitro evaluation of paclitaxel loaded amorphous chitin nanoparticles for colon cancer drug delivery

Author:
Smitha, K.T., Anitha, A., Furuike, T., Tamura, H., Nair, Shantikumar V., Jayakumar, R.
Source:
Colloids and Surfaces B: Biointerfaces 2013 v.104 pp. 245-253
ISSN:
0927-7765
Subject:
antineoplastic agents, biodegradability, chitin, colloids, colorectal neoplasms, crosslinking, drug delivery systems, drug therapy, flow cytometry, fluorescence microscopy, in vitro studies, nanoparticles, neoplasm cells, paclitaxel, toxicity
Abstract:
Chitin and its derivatives have been widely used in drug delivery applications due to its biocompatible, biodegradable and non-toxic nature. In this study, we have developed amorphous chitin nanoparticles (150±50nm) and evaluated its potential as a drug delivery system. Paclitaxel (PTX), a major chemotherapeutic agent was loaded into amorphous chitin nanoparticles (AC NPs) through ionic cross-linking reaction using TPP. The prepared PTX loaded AC NPs had an average diameter of 200±50nm. Physico-chemical characterization of the prepared nanoparticles was carried out. These nanoparticles were proven to be hemocompatible and in vitro drug release studies showed a sustained release of PTX. Cellular internalization of the NPs was confirmed by fluorescent microscopy as well as by flow cytometry. Anticancer activity studies proved the toxicity of PTX-AC NPs toward colon cancer cells. These preliminary results indicate the potential of PTX-AC NPs in colon cancer drug delivery.
Agid:
957153