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22. Long-termed cryopreservation of bone marrow stem cells, peripheral lymphocytes, and umbilical cord cells in liquid nitrogen since 1972: Part-2
- Sumida, S., Kitamura, T., Motomura, N., Saito, A.
- Cryobiology 2012 v.65 no.3 pp. 345-346
- autografting, bone marrow, bone marrow cells, containers, cryopreservation, lymphocytes, nitrogen, patients, stem cells, umbilical cord, viability
- AIM: This study was performed to confirm viability and differentiation in culture of bone marrow cells cryopreserved since 1972. Materials and Methods were presented previously as part 1. RESULTS AND CONCLUSION: Bone marrow cells from 293 advanced cancer patients were collected and frozen in 1972 for allogeneic and after 1982 for autologous transplantation. Containers with bone marrow which were not re-infused into patients were continuously preserved in a liquid nitrogen stock.