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PU.1 directly regulates retinoic acid-induced expression of RIG-G in leukemia cells

Author:
Gu, Zhi-Min, Liu, Chuan-Xu, Wu, Shao-Fang, Zhao, Meng, Xu, Han-Zhang, Liu, Wei, Zhou, Hu-Chen, Chen, Guo-Qiang, Wu, Ying-Li
Source:
FEBS letters 2011 v.585 no.2 pp. 375-380
ISSN:
0014-5793
Subject:
RNA, binding sites, gel electrophoresis, gene expression regulation, genes, interferons, leukemia, luciferase, retinoic acid
Abstract:
RIG-G is a retinoic acid- or interferon-induced gene with potential anti-proliferation function. However, the mechanism underlying ATRA-induced RIG-G induction is not completely understood. Here, we demonstrate that ATRA up-regulates the expression of PU.1, which in turn directly binds to the promoter and increases the expression of RIG-G gene. Luciferase reporter assay and electrophoretic mobility shift assay reveal that PU.1 preferentially binds to one of the two putative binding sites on the RIG-G promoter. Moreover, silencing of PU.1 by shRNA markedly inhibited ATRA- but not IFNα-induced expression of RIG-G. These data provide new insight into the mechanism of ATRA-induced RIG-G expression.
Agid:
982873