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Further studies and biological activities of macromolecular protein R-Phycoerythrin from Portieria hornemannii

Author:
Senthilkumar, Namasivayam, Kurinjimalar, Chidambaram, Thangam, Ramar, Suresh, Veeraperumal, Kavitha, Ganapathy, Gunasekaran, Palani, Rengasamy, Ramasamy
Source:
International journal of biological macromolecules 2013 v.62 pp. 107-116
ISSN:
0141-8130
Subject:
DNA fragmentation, EDTA (chelating agent), Rhodophyta, acetone, antioxidant activity, apoptosis, cell cycle, chloroform, citric acid, denaturation, ethanol, ethyl acetate, fluorescence, human cell lines, methanol, nuclear genome, shrinkage, solvents, thermal degradation, weight loss
Abstract:
In the present study, the purified R-Phycoerythrin (R-PE) from a red alga Portieria hornemannii was subjected to the analysis of stability under the influence of different agents. Among the various inhibitors tested on R-PE EDTA at lower concentrations (<1mM) supported the activity of R-PE. When R-PE was exposed to different organic solvents, ethanol supported the activity at the maximum followed by acetone, ethyl acetate, chloroform and methanol. Citric acid, as a preservative maintained the stability of R-PE both under 0±5°C and 30±5°C with 59.34% and 56.23% respectively, on 30th day. Thermal decomposition of the R-PE began near 60°C. Maximum weight loss was occurred between 150°C and 500°C. Complete weight loss was recorded around 875°C. Thermal denaturation was observed between 19°C and 40°C. Moderate to low antioxidant activities were observed in R-PE in relation to total antioxidant activities. After characterization, R-PE was taken for in vitro anticancer studies against selected cancer cell lines. Further studies involving AO/EB fluorescence staining and phase contrast microscope revealed characteristic apoptotic features like cell shrinkage, membrane blebbing, and nuclear DNA fragmentation, etc. Likewise, FACS analysis revealed the cell cycle distribution pattern of A549 and HepG2 cells.
Agid:
997860